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Article

A Novel Assay Coupling Dephosphorylation and Blue/White Colony Screening for the G > A Hotspot Mutation at Codon 13 of KRAS Gene.

Journal of nanoscience and nanotechnology - 1 Jan 2018

Zhou Cuilan, Pu Wangyang, Yin Yu-Fang, Xiao Li, Luo Dixian, Wang Fenjiao, Peng Cuiying, Guo Zifen, Liu Xuan, Xu Huifen, Xing Chungen, He Nongyue, Sun Wanping, Cai Weijun, Liao Duanfang, Li Kai

Abstract excerpt

Development of sensitive assay for detection of hotspot mutations of cancer driving gene is crucial for circulating tumor DNA analysis. This study tested the possibilities of applying restriction enzyme digestion and dephosphorylation coupled with blue/white screening technology for analyzing a hotspot point mutation in codon 13 of KRAS gene. The present study has documented that the combination of PCR with...

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Identifiers

PMID: 29768879
DOI: 10.1166/jnn.2018.13935

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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