Article
Assay design and optimization of mutant-enriched PCR based method for detection of K-ras gene mutations in pancreatic carcinoma.
Neoplasma - 1 Jan 2006
Ugorcáková J, Hlavatý T, Babál P, Janega P, Bukovská G, Halgasová N, Hlavatá A, Timko J, Duris I
Abstract excerpt
The aim of our work was to develop a fast, reliable and sensitive PCR method to detect K-ras mutations in various clinical samples. There is a need for an unimpeachable method for early diagnosis and/or screening of pancreatic cancer (PC). We optimized and subsequently analyzed four methods based on mutant-enriched PCR for the sensitivity, cost and time expense. Using the selected optimal method we examined codon...
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