Article
Combinatorial Evolution of DNA with RECODE.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Kang Zhen, Ding Wenwen, Jin Peng, Du Guocheng, Chen Jian
Abstract excerpt
In past decades, DNA engineering protocols have led to the rapid development of synthetic biology. To engineer the natural proteins, many directed evolution methods based on molecular biology have been presented for generating genetic diversity or obtaining specific properties. Here, we provide a simple (PCR operation), efficient (larger amount of products), and powerful (multiple point mutations, deletions,...
Topics
- DNA
- DNA Ligases
- DNA-Directed DNA Polymerase
- Directed Molecular Evolution
- Gene Library
- Genetic Variation
- Mutagenesis
- Polymerase Chain Reaction
- Synthetic Biology