Molecular evolution of a defined DNA sequence with accumulation of mutations in a single round by a dual approach to random chemical mutagenesis (DuARCheM).

doi:10.1002/cbic.200800259

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Article

Molecular evolution of a defined DNA sequence with accumulation of mutations in a single round by a dual approach to random chemical mutagenesis (DuARCheM).

Chembiochem : a European journal of chemical biology - 22 Sept 2008

Mohan Utpal, Banerjee Uttam Chand

Abstract excerpt

Directed evolution has paved the way to a new era of protein and nucleic acid molecules with improved and enhanced properties. The utmost important component of directed evolution is random mutations in a defined DNA sequence. The utility of random chemical mutagenesis in directed evolution studies is dwindling due to the inherent flaws with whole-organism mutagenesis and the in vitro approach. Here, we report a...

Topics

Base Sequence
DNA
Directed Molecular Evolution
Ethyl Methanesulfonate
Hydrolysis
Incubators
Lipase
Mutagenesis
Mutagens
Mutation
Pseudomonas aeruginosa
Time Factors

Source

Open in PubMed

Identifiers

PMID: 18756549
DOI: 10.1002/cbic.200800259

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