Article
Targeted editing of tomato carotenoid isomerase reveals the role of 5' UTR region in gene expression regulation.
Plant cell reports - 1 Apr 2021
Lakshmi Jayaraj K, Thulasidharan Nitu, Antony Aju, John Moni, Augustine Rehna, Chakravartty Navajeet, Sukumaran Smitha, Uma Maheswari M, Abraham Sweety, Thomas George, Lachagari V B Reddy, Seshagiri Somasekar, Narayanan Subhash, Kuriakose Boney
Abstract excerpt
KEY MESSAGE: A deletion created by CRISPR/Cas9 system in the 5' UTR of the carotenoid isomerase gene in tomato leads to downregulation of the gene resulting in the low conversion of prolycopene to lycopene. CRISPR/Cas9 based genome editing is an effective and useful tool adopted from the bacterial immune response system for altering specific, pre-determined DNA sequences in eukaryotes. Such targeted changes are...
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