Article
CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations.
Scientific reports - 21 Apr 2016
Pan Changtian, Ye Lei, Qin Li, Liu Xue, He Yanjun, Wang Jie, Chen Lifei, Lu Gang
Abstract excerpt
The CRISPR/Cas9 system has successfully been used in various organisms for precise targeted gene editing. Although it has been demonstrated that CRISPR/Cas9 system can induce mutation in tomato plants, the stability of heredity in later generations and mutant specificity induced by the CRISPR/Cas9 system in tomato plants have not yet been elucidated in detail. In this study, two genes, SlPDS and SlPIF4, were used...
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