Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

pKAMA-ITACHI Vectors for Highly Efficient CRISPR/Cas9-Mediated Gene Knockout in Arabidopsis thaliana.

Plant & cell physiology - 1 Jan 2017

Tsutsui Hiroki, Higashiyama Tetsuya

Abstract excerpt

The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) system is widely used as a tool for genome engineering in various organisms. A complex consisting of Cas9 and single guide RNA (sgRNA) induces a DNA double-strand break in a sequence-specific manner, resulting in knockout. Some binary vectors for CRISPR/Cas9 in plants have been reported, but there is a problem with low...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Arabidopsis
Share to CRISPR-Cas Systems
Share to Gene Editing

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Knockout Techniques

Share to Genetic Vectors

Share to Plants, Genetically Modified

Share to Reproducibility of Results

Share to Sequence Homology, Nucleic Acid

Source

Open in PubMed Open full text

Identifiers

PMID: 27856772
DOI: 10.1093/pcp/pcw191
PMCID: PMC5444565

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

pKAMA-ITACHI Vectors for Highly Efficient CRISPR/Cas9-Mediated Gene Knockout in Arabidopsis thaliana.PMID 27856772Read ↗

Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis.Semantic proximity Read ↗

CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications.Semantic proximity Read ↗

CRISPR/Cas systems: opportunities and challenges for crop breeding.Semantic proximity Read ↗

Advancements in CRISPR-Mediated Multiplex Genome Editing: Transforming Plant Breeding for Crop Improvement and Polygenic Trait EngineeringSemantic proximity Read ↗

A dual sgRNA approach for functional genomics in <i>Arabidopsis thaliana</i> <sup>[OPEN]</sup>Semantic proximity Read ↗

Emerging applications of CRISPR-Cas9 genome editing in horticultural crop improvementSemantic proximity Read ↗

The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.Semantic proximity Read ↗

Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles.Semantic proximity Read ↗

Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis.Semantic proximity Read ↗

Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis.Semantic proximity Read ↗

CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases.Semantic proximity Read ↗

Exploiting the CRISPR/Cas9 System for Targeted Genome Mutagenesis in Petunia.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.