Article
CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses.
Molecular biology of the cell - 1 Oct 2018
Su Kuan-Chung, Tsang Mary-Jane, Emans Neil, Cheeseman Iain M
Abstract excerpt
A key goal for cell biological analyses is to assess the phenotypes that result from eliminating a target gene. Since the early 1990s, the predominant strategy utilized in human tissue culture cells has been RNA interference (RNAi)-mediated protein depletion. However, RNAi suffers well-documented off-target effects as well as incomplete and reversible protein depletion. The implementation of CRISPR/Cas9-based DNA...
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