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Article

High-throughput construction of multiple cas9 gene variants via assembly of high-depth tiled and sequence-verified oligonucleotides.

Nucleic acids research - 18 May 2018

Cho Namjin, Seo Han Na, Ryu Taehoon, Kwon Euijin, Huh Sunghoon, Noh Jinsung, Yeom Huiran, Hwang Byungjin, Ha Heejeong, Lee Ji Hyun, Kwon Sunghoon, Bang Duhee

Abstract excerpt

Selective retrieval of sequence-verified oligonucleotides (oligos) from next-generation sequencing (NGS) flow cells, termed megacloning, promises accurate and reliable gene synthesis. However, gene assembly requires a complete collection of overlapping sense and nonsense oligos, and megacloning does not typically guarantee the complete production of sequence-verified oligos. Therefore, missing oligos must be...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 29529247
DOI: 10.1093/nar/gky112
PMCID: PMC5961255

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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