Article

Cas12a-Capture: a novel, low-cost, and scalable method for targeted sequencing

2020-11-20

Abstract excerpt

Targeted sequencing remains a valuable technique for clinical and research applications. However, many existing technologies suffer from pervasive GC sequence content bias, high input DNA requirements, and high cost for custom panels. We have developed Cas12a-Capture, a low-cost and highly scalable method for targeted sequencing. The method utilizes preprogramed guide RNAs to direct CRISPR-Cas12a cleavage of doubl...

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Literature Corpus work: 2d6d130e-2d6b-58e3-9120-67f8e0c91dd3
DOI: 10.1101/2020.11.18.388876

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