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Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering.

Nucleic acids research - 18 May 2018

Rasila Tiina S, Pulkkinen Elsi, Kiljunen Saija, Haapa-Paananen Saija, Pajunen Maria I, Salminen Anu, Paulin Lars, Vihinen Mauno, Rice Phoebe A, Savilahti Harri

Abstract excerpt

The phage Mu DNA transposition system provides a versatile species non-specific tool for molecular biology, genetic engineering and genome modification applications. Mu transposition is catalyzed by MuA transposase, with DNA cleavage and integration reactions ultimately attaching the transposon DNA to target DNA. To improve the activity of the Mu DNA transposition machinery, we mutagenized MuA protein and...

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PMID: 29294068
DOI: 10.1093/nar/gkx1281
PMCID: PMC5961161

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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