Article
Sequence and positional requirements for DNA sites in a mu transpososome.
The Journal of biological chemistry - 8 Mar 2002
Goldhaber-Gordon Ilana, Early Michael H, Gray Matthew K, Baker Tania A
Abstract excerpt
Transposition of bacteriophage Mu uses two DNA cleavage sites and six transposase recognition sites, with each recognition site divided into two half-sites. The recognition sites can activate transposition of non-Mu DNA sequences if a complete set of Mu sequences is not available. We have analyzed 18 sequences from a non-Mu DNA molecule, selected in a functional assay for the ability to be transposed by MuA...
Topics
- Bacteriophage phi X 174
- Base Sequence
- Binding Sites
- DNA
- DNA Transposable Elements
- Gene Deletion
- Models, Biological
- Molecular Sequence Data
- Mutation
- Protein Binding
- Protein Structure, Tertiary
- Sequence Homology, Nucleic Acid
- Transposases