Article
Effect of mutations in the Mu-host junction region on transpososome assembly.
Journal of molecular biology - 6 Jul 2001
Coros C J, Chaconas G
Abstract excerpt
Mu transposition occurs through a series of higher-order nucleoprotein complexes called transpososomes. The region where the Mu DNA joins the host DNA plays an integral role in the assembly of these transpososomes. We have created a series of point mutations at the Mu-host junction and characterized their effect on the Mu in vitro strand transfer reaction. Analysis of these mutant constructs revealed an...
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