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Article

Step-arrest mutants of phage Mu transposase. Implications in DNA-protein assembly, Mu end cleavage, and strand transfer.

The Journal of biological chemistry - 20 Jan 1995

Kim K, Namgoong S Y, Jayaram M, Harshey R M

Abstract excerpt

We describe the isolation and characterization of Mu A variants arrested at specific steps of transposition. Mutations at 13 residues within the Mu A protein were analyzed for precise excision of Mu DNA in vivo. A subset of the defective variants (altered at Asp269, Asp294, Gly348, and Glu392) were tested in specific steps of transposition in vitro. It is possible that at least some residues of the...

Topics

Amino Acid Sequence
Bacteriophage mu
Calcium
DNA, Viral
Hydrolysis
Ions
Magnesium
Manganese
Molecular Sequence Data
Mutation
Nucleotidyltransferases
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sequence Homology, Amino Acid

Synapses

Transposases

Source

Identifiers

PMID: 7836417
DOI: 10.1074/jbc.270.3.1472

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7836417-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Step-arrest mutants of phage Mu transposase. Implications in DNA-protein assembly, Mu end cleavage, and strand transfer.PMID 7836417Read ↗

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