Article
Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity.
Nature communications - 12 Dec 2017
Winters Ian P, Chiou Shin-Heng, Paulk Nicole K, McFarland Christopher D, Lalgudi Pranav V, Ma Rosanna K, Lisowski Leszek, Connolly Andrew J, Petrov Dmitri A, Kay Mark A, Winslow Monte M
Abstract excerpt
Large-scale genomic analyses of human cancers have cataloged somatic point mutations thought to initiate tumor development and sustain cancer growth. However, determining the functional significance of specific alterations remains a major bottleneck in our understanding of the genetic determinants of cancer. Here, we present a platform that integrates multiplexed AAV/Cas9-mediated homology-directed repair (HDR)...
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