Article
A prime editor mouse to model a broad spectrum of somatic mutations in vivo.
Nature biotechnology - 1 Mar 2024
Ely Zackery A, Mathey-Andrews Nicolas, Naranjo Santiago, Gould Samuel I, Mercer Kim L, Newby Gregory A, Cabana Christina M, Rideout William M, Jaramillo Grissel Cervantes, Khirallah Jennifer M, Holland Katie, Randolph Peyton B, Freed-Pastor William A, Davis Jessie R, Kulstad Zachary, Westcott Peter M K, Lin Lin, Anzalone Andrew V, Horton Brendan L, Pattada Nimisha B, Shanahan Sean-Luc, Ye Zhongfeng, Spranger Stefani, Xu Qiaobing, Sánchez-Rivera Francisco J, Liu David R, Jacks Tyler
Abstract excerpt
Genetically engineered mouse models only capture a small fraction of the genetic lesions that drive human cancer. Current CRISPR-Cas9 models can expand this fraction but are limited by their reliance on error-prone DNA repair. Here we develop a system for in vivo prime editing by encoding a Cre-inducible prime editor in the mouse germline. This model allows rapid, precise engineering of a wide range of mutations...
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