Article
CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and Functional Analysis of Oncogenic Driver Mutations.
Molecular cell - 4 Aug 2016
Guernet Alexis, Mungamuri Sathish Kumar, Cartier Dorthe, Sachidanandam Ravi, Jayaprakash Anitha, Adriouch Sahil, Vezain Myriam, Charbonnier Françoise, Rohkin Guy, Coutant Sophie, Yao Shen, Ainani Hassan, Alexandre David, Tournier Isabelle, Boyer Olivier, Aaronson Stuart A, Anouar Youssef, Grumolato Luca
Abstract excerpt
Intratumor genetic heterogeneity underlies the ability of tumors to evolve and adapt to different environmental conditions. Using CRISPR/Cas9 technology and specific DNA barcodes, we devised a strategy to recapitulate and trace the emergence of subpopulations of cancer cells containing a mutation of interest. We used this approach to model different mechanisms of lung cancer cell resistance to EGFR inhibitors and...
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