Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote.

Scientific reports - 17 Feb 2017

Raveux Aurélien, Vandormael-Pournin Sandrine, Cohen-Tannoudji Michel

Abstract excerpt

Microinjection of the CRISPR/Cas9 system in zygotes is an efficient and comparatively fast method to generate genetically modified mice. So far, only few knock-in mice have been generated using this approach, and because no systematic study has been performed, parameters controlling the efficacy of CRISPR/Cas9-mediated targeted insertion are not fully established. Here, we evaluated the effect of several...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Clustered Regularly Interspaced Short Palindromic Repeats
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Endonucleases

Share to Gene Knock-In Techniques

Share to Microinjections

Share to Oligodeoxyribonucleotides

Share to RNA, Guide, CRISPR-Cas Systems

Share to Zygote

Source

Open in PubMed Open full text

Identifiers

PMID: 28209967
DOI: 10.1038/srep42661
PMCID: PMC5314402

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote.PMID 28209967Read ↗

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice.Semantic proximity Read ↗

Electroporation and genetic supply of Cas9 increase the generation efficiency of CRISPR/Cas9 knock-in alleles in C57BL/6J mouse zygotes.Semantic proximity Read ↗

Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein.Semantic proximity Read ↗

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes.Semantic proximity Read ↗

Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering.Semantic proximity Read ↗

Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.Semantic proximity Read ↗

Generation of Genetically Modified Mice through the Microinjection of Oocytes.Semantic proximity Read ↗

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.Semantic proximity Read ↗

Pervasive Genotypic Mosaicism in Founder Mice Derived from Genome Editing through Pronuclear Injection.Semantic proximity Read ↗

Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology.Semantic proximity Read ↗

Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing.Semantic proximity Read ↗

Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.