Article
Studying Topoisomerase 1-Mediated Damage at Genomic Ribonucleotides.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Williams Jessica S, Kunkel Thomas A
Abstract excerpt
Ribonucleotides incorporated into DNA by the DNA polymerases can be incised by Topoisomerase 1 (Top1) to initiate removal of ribonucleotides from the genome. This Top1-dependent ribonucleotide removal has been demonstrated to result in multiple forms of genome instability in yeast. Here, we describe both quantitative and qualitative assays to identify mutations and other forms of DNA damage resulting from...
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