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Article

Disruption of diphthamide synthesis genes and resulting toxin resistance as a robust technology for quantifying and optimizing CRISPR/Cas9-mediated gene editing.

Scientific reports - 13 Nov 2017

Killian Tobias, Dickopf Steffen, Haas Alexander K, Kirstenpfad Claudia, Mayer Klaus, Brinkmann Ulrich

Abstract excerpt

We have devised an effective and robust method for the characterization of gene-editing events. The efficacy of editing-mediated mono- and bi-allelic gene inactivation and integration events is quantified based on colony counts. The combination of diphtheria toxin (DT) and puromycin (PM) selection enables analyses of 10,000-100,000 individual cells, assessing hundreds of clones with inactivated genes per...

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Identifiers

PMID: 29133816
DOI: 10.1038/s41598-017-15206-x
PMCID: PMC5684134

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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