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Detection and Modulation of DNA Translocations During Multi-Gene Genome Editing in T Cells

1 Jun 2020

Abstract excerpt

Multiplexed genome editing with DNA endonucleases has broad application, including for cellular therapies, but chromosomal translocations, natural byproducts of inducing simultaneous genomic breaks, have not been explored in detail. Here we apply various CRISPR-Cas nucleases to edit the T cell receptor alpha and beta 2 microglobulin genes in human primary T cells and comprehensively evaluate the frequency and...

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PMID: 32584143
DOI: 10.1089/crispr.2019.0074

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