Article
Universal toxin-based selection for precise genome engineering in human cells.
Nature communications - 21 Jan 2021
Li Songyuan, Akrap Nina, Cerboni Silvia, Porritt Michelle J, Wimberger Sandra, Lundin Anders, Möller Carl, Firth Mike, Gordon Euan, Lazovic Bojana, Sieńska Aleksandra, Pane Luna Simona, Coelho Matthew A, Ciotta Giovanni, Pellegrini Giovanni, Sini Marcella, Xu Xiufeng, Mitra Suman, Bohlooly-Y Mohammad, Taylor Benjamin J M, Sienski Grzegorz, Maresca Marcello
Abstract excerpt
Prokaryotic restriction enzymes, recombinases and Cas proteins are powerful DNA engineering and genome editing tools. However, in many primary cell types, the efficiency of genome editing remains low, impeding the development of gene- and cell-based therapeutic applications. A safe strategy for robust and efficient enrichment of precisely genetically engineered cells is urgently required. Here, we screen for...
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