Article
Easy quantification of template-directed CRISPR/Cas9 editing.
Nucleic acids research - 1 Jun 2018
Brinkman Eva K, Kousholt Arne N, Harmsen Tim, Leemans Christ, Chen Tao, Jonkers Jos, van Steensel Bas
Abstract excerpt
Template-directed CRISPR/Cas9 editing is a powerful tool for introducing subtle mutations in genomes. However, the success rate of incorporation of the desired mutations at the target site is difficult to predict and therefore must be empirically determined. Here, we adapted the widely used TIDE method for quantification of templated editing events, including point mutations. The resulting TIDER method is a...
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