Article
Visualizing Soluble Protein Mutants by Using Monomeric Red Fluorescent Protein as a Reporter for Directed Evolution.
Applied biochemistry and biotechnology - 1 May 2018
Wang Xueying, Wang Lei, Lin Xinping, Yang Xiaobing, Liu Wujun, Zhao Zongbao K
Abstract excerpt
Directed evolution-based protein engineering usually generates large library contained insoluble mutants because of structural disturbance by mutation. To reduce the workload and costs, it is crucial to identify and eliminate those insoluble variants prior to dedicated analysis. Here, we demonstrate a method to visualize soluble protein mutants by using monomeric red fluorescent protein (mRFP) as a fusion tag. A...
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