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A Brief Guide to the High-Throughput Expression of Directed Evolution Libraries.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Ribeiro Ana Luísa, Mencía Mario, Hidalgo Aurelio

Abstract excerpt

The process of protein production optimization requires time and labor, constituting one of the main bottlenecks for the downstream utilization of the proteins. However, once through this bottleneck, the protein production process can be easily standardized and multiplexed to find the fittest variants in large libraries created by random mutagenesis. In this chapter, we present an overview of the most important...

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PMID: 29086306
DOI: 10.1007/978-1-4939-7366-8_7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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