Article
Red fluorescent proteins engineered from green fluorescent proteins.
Proceedings of the National Academy of Sciences of the United States of America - 7 Nov 2023
Imamura Hiromi, Otsubo Shiho, Nishida Mizuho, Takekawa Norihiro, Imada Katsumi
Abstract excerpt
Fluorescent proteins (FPs) form a fluorophore through autocatalysis from three consecutive amino acid residues within a polypeptide chain. The two major groups, green FPs (GFPs) and red FPs (RFPs), have distinct fluorophore structures; RFPs have an extended π-conjugation system with an additional double bond. However, due to the low sequence homology between the two groups, amino acid residues essential for...
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