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Article

Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution: creation and characterization of highly in vivo soluble mutants.

Protein engineering, design & selection : PEDS - 1 Aug 2011

Asano Yasuhisa, Dadashipour Mohammad, Yamazaki Mizue, Doi Nobutaka, Komeda Hidenobu

Abstract excerpt

Low protein solubility of recombinantly expressed proteins in Escherichia coli is a major factor hindering their application and analysis. We generated highly in vivo soluble mutants of a hydroxynitrile lyase in E.coli using protein engineering. Structure-guided saturation mutagenesis caused high...

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Identifiers

PMID: 21729945
DOI: 10.1093/protein/gzr030

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-21729945-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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