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A paper-based whole-cell screening assay for directed evolution-driven enzyme engineering.

Applied microbiology and biotechnology - 1 Jul 2020

Gul Ijaz, Bogale Tadesse Fantaye, Chen Yong, Yang Xian, Fang Ruiqin, Feng Juan, Gao Hui, Tang Lixia

Abstract excerpt

Directed evolution has become an important method to unleash the latent potential of enzymes to make them uniquely suited for human purposes. However, the need for a large reagent volume and sophisticated instrumentation hampers its broad implementation. In an attempt to address this problem, here we report a paper-based high-throughput screening approach that should find broad application in generating desired...

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PMID: 32367311
DOI: 10.1007/s00253-020-10615-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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