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Article

A simple dual selection for functionally active mutants of Plasmodium falciparum dihydrofolate reductase with improved solubility.

Protein engineering, design & selection : PEDS - 1 Oct 2005

Japrung D, Chusacultanachai S, Yuvaniyama J, Wilairat P, Yuthavong Y

Abstract excerpt

Sufficient solubility of the active protein in aqueous solution is a prerequisite for crystallization and other structural studies of proteins. In this study, we have developed a simple and effective in vivo screening system to select for functionally active proteins with increased solubility by using Plasmodium falciparum dihydrofolate reductase (pfDHFR), a well-known malarial drug target, as a model. Prior to...

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Identifiers

PMID: 16120637
DOI: 10.1093/protein/gzi044

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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