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Engineering a bright near-infrared fluorescent protein by screening a comprehensive phenotypic landscape.

Protein science : a publication of the Protein Society - 1 Jun 2026

Kulhanek Devon L, Wei Qiyao, Head Jared, Hellinger Jessica, Jansen Zachary, Gilmour Andrew R, Segall-Shapiro Thomas, Brodbelt Jennifer S, Usama Syed Muhammad, Thyer Ross

Abstract excerpt

Near-infrared fluorescent proteins (niRFPs) are valuable markers for tracking cellular phenomena as they offer greater imaging depth, lower background, and minimal invasiveness relative to other fluorescent probes. The small ultrared fluorescent protein (smURFP) is the brightest niRFP currently reported and has been the subject of several mutagenesis studies to improve its biophysical characteristics. Here, we...

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PMID: 42089709
DOI: 10.1002/pro.70610
PMCID: PMC13147947

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