Article
Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteria.
FEBS letters - 25 Sept 2003
Sörensen Meike, Lippuner Christoph, Kaiser Toralf, Misslitz Ana, Aebischer Toni, Bumann Dirk
Abstract excerpt
A rapidly maturing variant of the red fluorescent protein DsRed was optimized for bacterial expression by random mutagenesis. The brightest variant contains six mutations, two of which (S4T and a silent mutation in codon 2) explain most of the fluorescence enhancement. The novel variants are expressed at 9-60-fold higher levels in Escherichia coli compared to DsRed.T3, but are not superior fluorophores on a per...
Topics
- Animals
- Base Sequence
- DNA, Recombinant
- Escherichia coli
- Female
- Fluorescence
- Genetic Variation
- HeLa Cells
- Humans
- Leishmania mexicana
- Luminescent Proteins