Article
A practical teaching course in directed protein evolution using the green fluorescent protein as a model.
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology - 1 Jan 2000
Ruller Roberto, Silva-Rocha Rafael, Silva Artur, Cruz Schneider Maria Paula, Ward Richard John
Abstract excerpt
Protein engineering is a powerful tool, which correlates protein structure with specific functions, both in applied biotechnology and in basic research. Here, we present a practical teaching course for engineering the green fluorescent protein (GFP) from Aequorea victoria by a random mutagenesis strategy using error-prone polymerase chain reaction. Screening of bacterial colonies transformed with random mutant...
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