Article
Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells.
The EMBO journal - 15 Dec 2017
Schimmel Joost, Kool Hanneke, van Schendel Robin, Tijsterman Marcel
Abstract excerpt
Cells employ potentially mutagenic DNA repair mechanisms to avoid the detrimental effects of chromosome breaks on cell survival. While classical non-homologous end-joining (cNHEJ) is largely error-free, alternative end-joining pathways have been described that are intrinsically mutagenic. Which end-joining mechanisms operate in germ and embryonic cells and thus contribute to heritable mutations found in...
Topics
- Animals
- CRISPR-Cas Systems
- Cell Line
- DNA End-Joining Repair
- DNA Polymerase beta
- DNA-Directed DNA Polymerase
- Embryonic Stem Cells
- Hypoxanthine Phosphoribosyltransferase
- Mice
- Models, Genetic
- Mutation
- DNA Polymerase theta