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Article

In situ genotyping of a pooled strain library after characterizing complex phenotypes.

Molecular systems biology - 17 Oct 2017

Lawson Michael J, Camsund Daniel, Larsson Jimmy, Baltekin Özden, Fange David, Elf Johan

Abstract excerpt

In this work, we present a proof-of-principle experiment that extends advanced live cell microscopy to the scale of pool-generated strain libraries. We achieve this by identifying the genotypes for individual cells in situ after a detailed characterization of the phenotype. The principle is demonstrated by single-molecule fluorescence time-lapse imaging of Escherichia coli strains harboring barcoded plasmids that...

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Identifiers

PMID: 29042431
DOI: 10.15252/msb.20177951
PMCID: PMC5658705

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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