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Article

Time-resolved imaging-based CRISPRi screening.

Nature methods - 1 Jan 2020

Camsund Daniel, Lawson Michael J, Larsson Jimmy, Jones Daniel, Zikrin Spartak, Fange David, Elf Johan

Abstract excerpt

Our ability to connect genotypic variation to biologically important phenotypes has been seriously limited by the gap between live-cell microscopy and library-scale genomic engineering. Here, we show how in situ genotyping of a library of strains after time-lapse imaging in a microfluidic device overcomes this problem. We determine how 235 different CRISPR interference knockdowns impact the coordination of the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 31740817
DOI: 10.1038/s41592-019-0629-y

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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