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Article

A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level.

Genome biology - 28 May 2012

Binder Stephan, Schendzielorz Georg, Stäbler Norma, Krumbach Karin, Hoffmann Kristina, Bott Michael, Eggeling Lothar

Abstract excerpt

We present a novel method for visualizing intracellular metabolite concentrations within single cells of Escherichia coli and Corynebacterium glutamicum that expedites the screening process of producers. It is based on transcription factors and we used it to isolate new L-lysine producing mutants of C. glutamicum from a large library of mutagenized cells using fluorescence-activated cell sorting (FACS). This...

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Identifiers

PMID: 22640862
DOI: 10.1186/gb-2012-13-5-r40
PMCID: PMC3446293

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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