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Article

High-throughput, image-based screening of pooled genetic-variant libraries.

Nature methods - 1 Dec 2017

Emanuel George, Moffitt Jeffrey R, Zhuang Xiaowei

Abstract excerpt

We report a high-throughput screening method that allows diverse genotypes and corresponding phenotypes to be imaged in individual cells. We achieve genotyping by introducing barcoded genetic variants into cells as pooled libraries and reading the barcodes out using massively multiplexed fluorescence in situ hybridization. To demonstrate the power of image-based pooled screening, we identified brighter and more...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29083401
DOI: 10.1038/nmeth.4495
PMCID: PMC5958624

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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