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Assessing a single targeted next generation sequencing for human leukocyte antigen typing protocol for interoperability, as performed by users with variable experience.

Human immunology - 1 Oct 2017

Gandhi Manish J, Ferriola Deborah, Lind Curt, Duke Jamie L, Huynh Anh, Papazoglou Anna, Mackiewicz Kate, Christiansen Mette, Dong Wei, Hsu Susan, Thomas Dawn, Schneider Brittany, Pierce Erin, Kearns Jane, Kamoun Malek, Monos Dimitri, Askar Medhat

Abstract excerpt

BACKGROUND: A simplified protocol for HLA-typing -by NGS, developed for use with the Illumina MiSeq, was performed by technologists with varying NGS experience to assess accuracy and reproducibility. METHODS: Technologists from six laboratories typed the same 16 samples at HLA-A, B, C, DRB1, and DQB1. The protocol includes long range PCR, library preparation, and paired-end 250bp sequencing. Two indexing...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28732721
DOI: 10.1016/j.humimm.2017.07.012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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