Article
Determining performance characteristics of an NGS-based HLA typing method for clinical applications.
HLA - 1 Mar 2016
Duke J L, Lind C, Mackiewicz K, Ferriola D, Papazoglou A, Gasiewski A, Heron S, Huynh A, McLaughlin L, Rogers M, Slavich L, Walker R, Monos D S
Abstract excerpt
This study presents performance specifications of an in-house developed human leukocyte antigen (HLA) typing assay using next-generation sequencing (NGS) on the Illumina MiSeq platform. A total of 253 samples, previously characterized for HLA-A, -B, -C, -DRB1 and -DQB1 were included in this study, which were typed at high-resolution using a combination of Sanger sequencing, sequence-specific primer (SSP) and...
Topics
- Alleles
- DNA Primers
- DNA Probes
- Genotype
- HLA Antigens
- High-Throughput Nucleotide Sequencing
- Histocompatibility Testing
- Humans
- Polymerase Chain Reaction
- Racial Groups
- Sensitivity and Specificity