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Article

HLA-genotyping of clinical specimens using Ion Torrent-based NGS.

Human immunology - 1 Dec 2015

Barone Jonathan C, Saito Katsuyuki, Beutner Karl, Campo Maria, Dong Wei, Goswami Chirayu P, Johnson Erica S, Wang Zi-Xuan, Hsu Susan

Abstract excerpt

We have evaluated and validated the NXType™ workflow (One Lambda, Inc.) and the accompanying TypeStream™ software on the Ion Torrent Next Generation Sequencing (NGS) platform using a comprehensive testing panel. The panel consisted of 285 genomic DNA (gDNA) samples derived from four major ethnic populations and contained 59 PT samples and 226 clinical specimens. The total number of alleles from the six loci...

Topics

Alleles
Computational Biology
Gene Library
Genetic Variation
Genotype
HLA Antigens
High-Throughput Nucleotide Sequencing
Histocompatibility Testing
Humans
Multiplex Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Reproducibility of Results

Sequence Analysis, DNA

Source

Identifiers

PMID: 26423535
DOI: 10.1016/j.humimm.2015.09.014

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-26423535-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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