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Rapid and Efficient Identification of Caenorhabditis elegans Legacy Mutations Using Hawaiian SNP-Based Mapping and Whole-Genome Sequencing.

G3 (Bethesda, Md.) - 4 Mar 2015

Jaramillo-Lambert Aimee, Fuchsman Abigail S, Fabritius Amy S, Smith Harold E, Golden Andy

Abstract excerpt

The production of viable embryos requires the coordination of many cellular processes, including protein synthesis, cytoskeletal reorganization, establishment of polarity, cell migration, cell division, and in Caenorhabditis elegans, eggshell formation. Defects in any of these processes can lead to embryonic lethality. We examined six temperature-sensitive mutants as well as one nonconditional mutant that were...

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PMID: 25740937
DOI: 10.1534/g3.115.017038
PMCID: PMC4426357

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