Article
CD59-deficient bone marrow erythroid cells from rats treated with procarbazine and propyl-nitrosourea have mutations in the Pig-a gene.
Environmental and molecular mutagenesis - 1 Oct 2020
Revollo Javier R, Dad Azra, Pearce Mason G, Mittelstaedt Roberta A, Casildo Andrea, Lapidus Rena G, Robison Timothy W, Dobrovolsky Vasily N
Abstract excerpt
Procarbazine (PCZ) and N-propyl-N-nitrosourea (PNU) are rodent mutagens and carcinogens. Both induce GPI-anchored marker-deficient mutant-phenotype red blood cells (RBCs) in the flow cytometry-based rat RBC Pig-a assay. In the present study, we traced the origin of the RBC mutant phenotype by analyzing Pig-a mutations in the precursors of RBCs, bone marrow erythroid cells (BMEs). Rats were exposed to a total of...
Topics
- Animals
- Antineoplastic Agents
- Bone Marrow Cells
- CD59 Antigens
- Male
- Membrane Proteins
- Mutation
- Nitrosourea Compounds
- Procarbazine
- Rats
- Rats, Inbred F344
- Rats, Sprague-Dawley