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Article

A New Method, "Reverse Yeast Two-Hybrid Array" (RYTHA), Identifies Mutants that Dissociate the Physical Interaction Between Elg1 and Slx5.

Genetics - 1 Jul 2017

Lev Ifat, Shemesh Keren, Volpe Marina, Sau Soumitra, Levinton Nelly, Molco Maya, Singh Shivani, Liefshitz Batia, Ben Aroya Shay, Kupiec Martin

Abstract excerpt

The vast majority of processes within the cell are carried out by proteins working in conjunction. The Yeast Two-Hybrid (Y2H) methodology allows the detection of physical interactions between any two interacting proteins. Here, we describe a novel systematic genetic methodology, "Reverse Yeast Two-Hybrid Array" (RYTHA), that allows the identification of proteins required for modulating the physical interaction...

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Identifiers

PMID: 28476868
DOI: 10.1534/genetics.117.200451
PMCID: PMC5500160

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PCNA

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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