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Article

Structural toggle in the RNaseH domain of Prp8 helps balance splicing fidelity and catalytic efficiency.

Proceedings of the National Academy of Sciences of the United States of America - 2 May 2017

Mayerle Megan, Raghavan Madhura, Ledoux Sarah, Price Argenta, Stepankiw Nicholas, Hadjivassiliou Haralambos, Moehle Erica A, Mendoza Senén D, Pleiss Jeffrey A, Guthrie Christine, Abelson John

Abstract excerpt

Pre-mRNA splicing is an essential step of eukaryotic gene expression that requires both high efficiency and high fidelity. Prp8 has long been considered the "master regulator" of the spliceosome, the molecular machine that executes pre-mRNA splicing. Cross-linking and structural studies place the RNaseH domain (RH) of Prp8 near the spliceosome's catalytic core and demonstrate that prp8 alleles that map to a 17-aa...

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Identifiers

PMID: 28416677
DOI: 10.1073/pnas.1701462114
PMCID: PMC5422793

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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