Article
Opposing classes of prp8 alleles modulate the transition between the catalytic steps of pre-mRNA splicing.
Nature structural & molecular biology - 1 Jun 2007
Liu Li, Query Charles C, Konarska Maria M
Abstract excerpt
The spliceosome is thought to undergo a conformational change between the two catalytic steps of precursor messenger RNA splicing, although the specific events in this transition are poorly understood. We previously proposed a two-state model of splicing in which the conformations required for the first and second steps are in competition. Here, we identify and characterize a class of prp8 mutants that suppress...
Topics
- Adenosine Triphosphatases
- Alleles
- DNA Primers
- Models, Molecular
- Mutation
- Protein Conformation
- RNA Helicases
- RNA Splicing
- RNA Splicing Factors
- RNA, Small Nuclear
- Ribonucleoprotein, U4-U6 Small Nuclear
- Ribonucleoprotein, U5 Small Nuclear
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Spliceosomes