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Article

Splicing proofreading at 5' splice sites by ATPase Prp28p.

Nucleic acids research - 1 Apr 2013

Yang Fei, Wang Xiu-Ye, Zhang Zhi-Min, Pu Jia, Fan Yu-Jie, Zhou Jiahai, Query Charles C, Xu Yong-Zhen

Abstract excerpt

Fidelity and efficiency of pre-mRNA splicing are critical for generating functional mRNAs, but how such accuracy in 5' splice site (SS) selection is attained is not fully clear. Through a series of yeast genetic screens, we isolated alleles of prp28 that improve splicing of suboptimal 5'SS substrates, demonstrating that WT-Prp28p proofreads, and consequently rejects, poor 5'SS. Prp28p is thought to facilitate the...

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Identifiers

PMID: 23462954
DOI: 10.1093/nar/gkt149
PMCID: PMC3632134

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Source receipt: pubmed, retrieved 7 Sept 2026.

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