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Article

Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.

Genes & development - 1 Aug 1999

Collins C A, Guthrie C

Abstract excerpt

The highly conserved spliceosomal protein Prp8 is known to cross-link the critical sequences at both the 5' (GU) and 3' (YAG) ends of the intron. We have identified prp8 mutants with the remarkable property of suppressing exon ligation defects due to mutations in position 2 of the 5' GU, and all positions of the 3' YAG. The prp8 mutants also suppress mutations in position A51 of the critical ACAGAG motif in U6...

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Identifiers

PMID: 10444595
DOI: 10.1101/gad.13.15.1970
PMCID: PMC316919

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Source receipt: pubmed, retrieved 7 Sept 2026.

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