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Article

Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system.

Nucleic acids research - 19 May 2017

Takayama Kazuo, Igai Keisuke, Hagihara Yasuko, Hashimoto Rina, Hanawa Morifumi, Sakuma Tetsushi, Tachibana Masashi, Sakurai Fuminori, Yamamoto Takashi, Mizuguchi Hiroyuki

Abstract excerpt

Genome editing research of human ES/iPS cells has been accelerated by clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) and transcription activator-like effector nucleases (TALEN) technologies. However, the efficiency of biallelic genetic engineering in transcriptionally inactive genes is still low, unlike that in transcriptionally active genes. To enhance the biallelic...

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Identifiers

PMID: 28334759
DOI: 10.1093/nar/gkx130
PMCID: PMC5435997

Genes

RAD51

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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