Article
A TALEN genome-editing system for generating human stem cell-based disease models.
Cell stem cell - 7 Feb 2013
Ding Qiurong, Lee Youn-Kyoung, Schaefer Esperance A K, Peters Derek T, Veres Adrian, Kim Kevin, Kuperwasser Nicolas, Motola Daniel L, Meissner Torsten B, Hendriks William T, Trevisan Marta, Gupta Rajat M, Moisan Annie, Banks Eric, Friesen Max, Schinzel Robert T, Xia Fang, Tang Alexander, Xia Yulei, Figueroa Emmanuel, Wann Amy, Ahfeldt Tim, Daheron Laurence, Zhang Feng, Rubin Lee L, Peng Lee F, Chung Raymond T, Musunuru Kiran, Cowan Chad A
Abstract excerpt
Transcription activator-like effector nucleases (TALENs) are a new class of engineered nucleases that are easier to design to cleave at desired sites in a genome than previous types of nucleases. We report here the use of TALENs to rapidly and efficiently generate mutant alleles of 15 genes in cultured somatic cells or human pluripotent stem cells, the latter for which we differentiated both the targeted lines...
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