Article
Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression.
Nature communications - 23 May 2017
Rosenbluh Joseph, Xu Han, Harrington William, Gill Stanley, Wang Xiaoxing, Vazquez Francisca, Root David E, Tsherniak Aviad, Hahn William C
Abstract excerpt
CRISPR-Cas9 provides the means to perform genome editing and facilitates loss-of-function screens. However, we and others demonstrated that expression of the Cas9 endonuclease induces a gene-independent response that correlates with the number of target sequences in the genome. An alternative approach to suppressing gene expression is to block transcription using a catalytically inactive Cas9 (dCas9). Here we...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.